Water is the most fundamental, essential and abundantly available natural resource. The analysis of water quality of the aquifers of important town areas of Thrissur district was carried out during the pre-monsoon period of 2014. 42 samples are collected from 13 sites. The physico – chemical and biological parameters such as Temperature, Turbidity, EC, TDS, pH, Alkalinity, Chloride, Salinity, TH, Ca, Mg, Iron, Phosphate, Sulphate, DO, COD, Total coliform count and E.coli count were determined. All the physico-chemical parameters of the sample are above the desirable limit but below the permissible limit. In this study all the 42 samples shows, higher amount of Coliform count beyond the permissible limit and presence of E.coli.